|
KMID : 1140120060110030240
|
|
Cancer Prevention Research
2006 Volume.11 No. 3 p.240 ~ p.247
|
|
|
Anti-proliferative Effects of Water Extract of Asarum sieboldii in Human Cancer Cells
|
|
Kim Ok-Suk
Park Cheol
Moon Sung-Gi
Choi Yung-Hyun
|
|
|
Abstract
|
|
|
|
Asarum sieboldii, as a medicinal and food plant, has been used to treat pain and inflammation in traditional Korean medicine. In the present study, it was analyzed the effects of water extracts of A. sieboldii (WEAS) on the growth and apoptosis of human lung carcinoma (A549 and NCI-H460) and colon cancer (HT29) cell lines. Treatment with WEAS in A549 cells inhibited the cell growth and induced apoptosis in a dose-dependent manner as measured by MTT assay, however NCI-H460 and HT29 cells exhibited relatively weak anti- proliferative activities to WEAS. RT-PCR and Western blot data revealed that the anti-proliferative effect of WEAS in A549 cells was associated with a dose-dependent up-regulation of cyclin-dependent kinase inhibitor p21 (WAF1/CIP1) protein in a tumor suppressor p53-independent fashion. However the levels of proliferating cell nuclear antigen (PCNA) mRNA and protein expression were markedly down-regulated by WEAS treatment. It was also found WEAS treatment decreased the levels of cyclooxygenase (COX)-2 and inducible nitric oxide synthease (iNOS) expression without significant changes in the expression of COX-1. Though further studies will be needed to identify the active compounds that confer the anti-cancer activity of WEAS, the present findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of A. sieboldii.
|
|
|
KEYWORD
|
|
|
Asarum sieboldii, p21, PCNA, COX-2, iNOS
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|